: The explant is moved to a medium enriched with hormones (often cytokinins like BAP) to stimulate the growth of multiple new shoots.
: The fragile plantlets are gradually transitioned from the high-humidity sterile environment to soil and natural light conditions. Essential Requirements Plant Propagation by Tissue Culture
: Individual shoots are transferred to a rooting medium, usually containing auxins (like IBA or NAA), to develop a healthy root system. : The explant is moved to a medium